Introduction: Determining sex-based differences in response to exercise is an important biological determinant of vulnerability to psychological and physiological stress. Previous studies have shown sex-based differences in basal salivary α-amylase activity, which is still controversial. The sex-based effects on salivary α-amylase activity following maximal cycling exercise remain to be clarified. The purpose of this study was to investigate the sex-based effects of maximal cycling exercise on salivary α-amylase activity in physically active men and women.