Physical activity can affect many aspects of metabolism but it is unclear to what extent this relies on manipulation of energy balance. Twenty‐six active men age 25 ± 7 years (mean ± SD) were randomly assigned either to consume 50% more energy than normal by over‐consuming their habitual diet for 7 days whilst simultaneously restricting their physical activity below 4000 steps day−1 to induce an energy surplus (SUR group; n= 14) or to the same regimen but with 45 min of daily treadmill running at 70% of maximum oxygen uptake (SUR+EX group; n= 12). Critically, the SUR+EX group received additional dietary energy intake to account for the energy expended by exercise, thus maintaining a matched energy surplus. At baseline and follow‐up, fasted blood samples and abdominal subcutaneous adipose tissue biopsies were obtained and oral glucose tolerance tests conducted. Insulinaemic responses to a standard glucose load increased 2‐fold from baseline to follow‐up in the SUR group (Δ17 ± 16 nmol (120 min) l−1; P= 0.002) whereas there was no change in the SUR+EX group (Δ1 ± 6 nmol (120 min) l−1). Seven of 17 genes within adipose tissue were differentially expressed in the SUR group; expression of SREBP‐1c, FAS and GLUT4 was significantly up‐regulated and expression of PDK4, IRS2, HSL and visfatin was significantly down‐regulated (P≤ 0.05). The pAMPK/AMPK protein ratio in adipose tissue was significantly down‐regulated in the SUR group (P= 0.005). Vigorous‐intensity exercise counteracted most of the effects of short‐term overfeeding and under‐activity at the whole‐body level and in adipose tissue, even in the face of a standardised energy surplus.

Direct Link: https://doi.org/10.1113/jphysiol.2013.262709

Journal: The Journal of physiology. 2013 Dec 15;591(24):6231-43

Keywords: energy balance, energy expenditure, nutrition, overfeeding, Physical Activity,

Applications: Energy Expenditure,

CamNtech Reference: AH13044

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