The Actiheart system (CamNtech Neurotechnology Ltd, Cambridge UK) is a small portable device that collects ECG derived heart rate and 3-dimensional accelerometer data, and uses these data to estimate body energy expenditure (BEE). High levels of accuracy for Actiheart based estimates of BEE have been reported over a range of daily activities. However, with the exception of treadmill walking, previous studies have used daily activities that are difficult to standardize and have poorly defined workloads. This study compared the accuracy of Actiheart based estimates of BEE during different types of standardized exercise with matching workloads under laboratory conditions.