Accelerometers are being increasingly used in studies of physical activity (PA) under a variety of circumstances, especially free-living environment. They can be used to assess a range of different aspects of PA, including energy expenditure, activity classification, gait, balance and fall. This paper reviews the use of accelerometers in these areas, along with the basic knowledge of accelerometers, preparatory work before data processing and the comparison of commonly-used products. The work of this review can provide a basis of accelerometer-used PA measurement and a contribution to further research and design.