Publications

CamNtech Research Library

 

CamNtech products have been trusted by specialist researchers for 25 years. Our research library contains peer reviewed publications citing the use of our devices. Filter by product, application, keyword, author or use free text search to locate your area of interest.

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Found 56 Results

Use of the Actiwatch-Mini® in dog safety studies as an early indicator for drug-induced behavioural changes

Introduction Clinical observations are routinely used to address drug-induced behavioural changes in dogs. To deal with the limitations of this methodology, we evaluated Actiwatch-Mini® as a tool to monitor continuously locomotor activity in dogs and to provide objective parameters that could be linked to behavioural changes after compound administration. Methods Eight Beagles were equipped with […]

Related Product: Actiwatch Mini

Publication Date: June 26, 2020


Age-related cognitive decline in baboons: modeling the prodromal phase of Alzheimer’s disease and related dementias

The aging of brain cells and synaptic loss are the major underlying pathophysiological processes contributing to the progressive decline in cognitive functions and Alzheimer’s disease. The difference in cognitive performances observed between adult and aged subjects across species highlights the decline of brain systems with age. The inflection point in age-related cognitive decline is important […]

Related Product: Actiwatch Mini

Publication Date: May 19, 2020


Behavioral and physiological processes in horses and their linkage with peripheral clock gene expression: A preliminary study

Behavioral and physiological processes have an innate 24-h cycle driven by the circadian master clock, which uses clock genes to generate rhythmicity and distribute temporal signals. Elucidating the blood gene expression in relation to the better known circadian rhythms in horses may contribute to improve the knowledge on the peripheral circadian rhythm control in this […]

Related Product: Actiwatch Mini

Publication Date: August 30, 2019


Eulerian videography technology improves classification of sleep architecture in primates

Sleep is a critically important dimension of primate behavior, ecology, and evolution, yet primate sleep is under-studied because current methods of analyzing sleep are expensive, invasive, and time-consuming. In contrast to electroencephalography (EEG) and actigraphy, videography is a cost-effective and non-invasive method to study sleep architecture in animals. With video data, however, it is challenging […]

Related Product: MotionWatch

Publication Date: August 27, 2019


Differences in dietary niche and foraging behavior of sympatric mule and white‐tailed deer

Mule (Odocoileus hemionus) and white‐tailed deer (Odocoileus virginianus) are congeneric and share similar life histories, yet their distribution is segregated across much of North America. Extensive research on both species within and outside their zone of co‐occurrence has not fully explained these distribution patterns, especially the potential role of diet and foraging behavior. Therefore, we […]

Related Product: MotionWatch

Publication Date: July 23, 2019


Environment shapes sleep patterns in a wild nocturnal primate

Among primates, the suborder Haplorhini is considered to have evolved a consolidated monophasic sleep pattern, with diurnal species requiring a shorter sleep duration than nocturnal species. Only a few primate species have been systematically studied in their natural habitat where environmental variables, including temperature and light, have a major influence on sleep and activity patterns. […]

Related Product: Actiwatch Mini

Publication Date: July 9, 2019


Sleep influences cognitive performance in lemurs

Primates spend almost half their lives asleep, yet little is known about how sleep influences their waking cognition. We hypothesized that diurnal and cathemeral lemurs differ in their need for consistent, non-segmented sleep for next-day cognitive function—including long-term memory consolidation, self-control, foraging efficiency, and sociality. Specifically, we expected that strictly diurnal Propithecus is more reliant […]

Related Product: MotionWatch

Publication Date: May 1, 2019


Cross-sectional comparison of health-span phenotypes in young versus geriatric marmosets.

The development of the marmoset as a translational model for healthspan and lifespan studies relies on the characterization of health parameters in young and geriatric marmosets. This cross-sectional study examined health phenotypes in marmosets for five domains of interest for human health and aging: mobility, cognition, metabolism, homeostasis, and immune function. Geriatric marmosets were found […]

Related Product: Actiwatch Mini

Publication Date: January 21, 2019


Determination of the pharmacokinetics of a single oral dose of trazodone and its effect on the activity level of domestic pigeons (Columba livia)

OBJECTIVE: To determine the pharmacokinetics of a single oral dose of trazodone and its effect on the activity of domestic pigeons (Columba livia). ANIMALS: 6 healthy adult male domestic pigeons. PROCEDURES: During the first of 3 experiments, birds received orally administered trazodone at doses ranging from 3 to 30 mg/kg to determine the dose for […]

Related Product: Actiwatch Mini

Publication Date: January 1, 2019


Chronobiology of voluntary food intake in reindeer (Rangifer tarandus tarandus)

n all organisms, biological rhythms arise from the interaction between endogenous clocks and a rhythmic environment. The timing of sunrise and sunset synchronize internal timing processes and inform the organism not only of the time of day, but also of the time of year. For animals living in polar regions biological timekeeping represents a challenge, […]

Related Product: MotionWatch

Publication Date: November 15, 2018


Circadian rhythms of melatonin and behaviour in juvenile sheep in field conditions: Effects of photoperiod, environment and weaning

Entrainment of circadian rhythms (CR) to the light dark cycle has been well described under controlled, experimental conditions. However, studies in rodents have reported that rhythms in the laboratory are not always reproduced under field conditions. The aim of this study was to characterise the CR of sheep maintained under conditions of standard UK farm […]

Related Product: Actiwatch Mini

Publication Date: October 1, 2018


Charting the onset of Parkinson-like motor and non-motor symptoms in nonhuman primate model of Parkinson’s disease

Parkinson’s disease is a progressive neurodegenerative disease increasingly affecting our aging population. Remarkable advances have been made in developing novel therapies to control symptoms, halt or cure the disease, ranging from physiotherapy and small molecules to cell and gene therapy. This progress was enabled by the existence of reliable animal models. The nonhuman primate model […]

Related Product: Actiwatch Mini

Publication Date: August 23, 2018


Ovarian estradiol supports sexual behavior but not energy homeostasis in female marmoset monkeys

Objective In adult female rodents, ovarian estradiol (E2) regulates body weight, adiposity, energy balance, physical activity, glucose-insulin homeodynamics, and lipid metabolism, while protecting against diet-induced obesity. The same E2 actions are presumed to occur in primates, but confirmatory studies have been lacking. Methods We investigated the consequences of ovariectomy (OVX) and E2 replacement in female […]

Related Product: Actiwatch Mini

Publication Date: July 18, 2018


The cost of deep sleep: Environmental influences on sleep regulation are greater for diurnal lemurs

Objectives Primates spend almost half their lives asleep, yet we know little about how evolution has shaped variation in the duration or intensity of sleep (i.e., sleep regulation) across primate species. Our objective was to test hypotheses related to how sleeping site security influences sleep intensity in different lemur species. Methods We used actigraphy and […]

Related Product: MotionWatch

Publication Date: July 10, 2018


The quest for welfare-friendly feeding of broiler breeders: effects of daily vs. 5: 2 feed restriction schedules

Restricted feeding of broiler breeders is required for improved long-term health and welfare. Because feeding frustration and hunger are major welfare concerns during rearing, many suggestions have been made to decrease the negative feelings of hunger while maintaining suitable growth rates and reproductive health. Non-daily (“skip-a-day”) feeding schedules are commonly used around the world to […]

Related Product: MotionWatch

Publication Date: December 7, 2017


Activity patterns in seven captive lemur species: Evidence of cathemerality in Varecia and Lemur catta?

Cathemerality, or activity throughout the 24‐hr cycle, is rare in primates yet relatively common among lemurs. However, the diverse ecological conditions under which cathemerality is expressed complicates attempts to identify species‐typical behavior. For example, Lemur catta and Varecia have historically been described as diurnal, yet recent studies suggest that they might exhibit cathemeral behavior under […]

Related Product: MotionWatch

Publication Date: March 3, 2017


Effects of 20-day litter weight on weaned piglets’ fighting behavior after group mixing and on heart rate variability in an isolation test.

Objective The objective of this study was to investigate the effect of 20-day litter weight on behavior and heart rate variability (HRV) of piglets under stress. Methods Forty four original litters were categorized as high litter weight (HW) litters (n = 22) and low litter weight (LW) litters (n = 22) by 20-day litter weight. […]

Related Product: Actiheart

Publication Date: February 1, 2017


The seasonal feeding ecology of the Javan slow loris (Nycticebus javanicus)

Objectives To describe the strategy employed by exudativorous primates during seasonal shifts in food abundance using the Javan slow loris as a model. Males and females may cope differently as well as exploit fallback foods in different proportions. Materials and methods Observing 15 free ranging Javan slow lorises over a year, we quantified their seasonal […]

Related Product: Actiwatch Mini

Publication Date: January 24, 2017


Eye and ear temperature using infrared thermography are related to rectal temperature in dogs at rest or with exercise

Rectal body temperature (BT) has been documented in exercising dogs to monitor thermoregulation, heat stress risk, and performance during physical activity. Eye (BTeye) and ear (BTear) temperature measured with infrared thermography (IRT) were compared to rectal (BTrec) temperature as the reference method and assess alternative sites to track hyperthermia, possibly to establish BTeye IRT as […]

Related Product: MotionWatch

Publication Date: December 19, 2016


VX toxicity in the Göttingen minipig.

The present experiments determined the intramuscular LD50 of VX in male Göttingen minipigs at two stages of development. In pubertal animals (115 days old), the LD50 of VX was indeterminate, but approximated 33.3 μg/kg. However, in sexually mature animals (152 days old), the LD50 was estimated to be only 17.4 μg/kg. Signs of nerve agent toxicity in […]

Related Product: MotionWatch

Publication Date: December 15, 2016


Higher fear of predators does not decrease outdoor range use in free-range Rowan Ranger broiler chickens

Organic chicken production is currently experiencing a boom in Sweden, but has only recently gained access to a commercial slow-growing broiler strain, the Rowan Ranger. While these chickens are reported to grow at rates better suited to organic production than previously available strains, anecdotal evidence suggested increased fearfulness in this strain and concerns were raised […]

Related Product: MotionWatch

Publication Date: June 23, 2016


The Rowan Ranger Chicken Breed-a Suitable Alternative for the Organic Chicken Meat Industry

The only available broiler strain to use in organic chicken meat production, until recently, has been conventional fast growing broiler hybrids. The Rowan Ranger is a broiler strain that has a naturally slower growth rate which makes this strain suitable for organic farming, meeting the demands of KRAV without being subjected to a feed restriction. […]

Related Product: MotionWatch

Publication Date: June 14, 2016


Physiological stress and behavioural differences in broiler breeder hens subjected to daily and 5: 2 feed restriction regimens

Feed restriction in the parental stock of meat producing chickens, broiler breeders, is essential in order to decrease the high growth rate that they are genetically selected for. The feed restriction does however lead to chronic hunger and stress. Stress can be measured by counting H/L ratios and the method of manually counting H/L ratios […]

Related Product: MotionWatch

Publication Date: June 9, 2016


Intrasubject and intersubject variabilities in the daily rhythm of total locomotor activity in horses

The observation of locomotor activity and resting behavior is used to determine time budgets, which may give some indication for the assessment of horses’ welfare concerning management and housing. The aim of this study was to investigate total locomotor activity (TLA) daily rhythm in athletic horses (Thoroughbred, Standardbred, and Italian Saddle), with special attention to […]

Related Product: Actiwatch Mini

Publication Date: February 13, 2016


Causal link of total locomotor activity, melatonin and rectal temperature daily rhythm in small ruminants

To improve the knowledge in chronophysiology we investigated the causal link between the most important physiological variable studied until now; ten Sarda ewes and ten Sarda goats, pluriparus not pregnant and no lactating, were used. Animals were housed under natural environmental conditions in a common stall, alfalfa hay and water were available ad libitum. Each […]

Related Product: Actiwatch Mini

Publication Date: February 7, 2016


Metabolic consequences of long-term rapamycin exposure on common marmoset monkeys (Callithrix jacchus).

Rapamycin has been shown to extend lifespan in rodent models, but the effects on metabolic health and function have been widely debated in both clinical and translational trials. Prior to rapamycin being used as a treatment to extend both lifespan and healthspan in the human population, it is vital to assess the side effects of […]

Related Product: Actiwatch Mini

Publication Date: November 13, 2015


Monitoring of total locomotor activity in mares during the prepartum and postpartum period

The aim of this study was to investigate the daily rhythms of total locomotor activity (TLA) in mares during the prepartum and postpartum periods and the existence of these rhythms at the time of birth in neonatal foals. For this purpose, TLA was recorded in 9 mares starting from the third day preceding the date […]

Related Product: Actiwatch Mini

Publication Date: June 16, 2015


Characterization of long-term motor deficits in the 6-OHDA model of Parkinson’s disease in the common marmoset

Research aimed at developing new therapies for Parkinson’s disease (PD) critically depend on valid animal models of the disease that allows for repeated testing of motor disabilities over extended time periods. We here present an extensive characterization of a wide range of motor symptoms in the 6-OHDA marmoset model of PD when tested over several […]

Related Product: Actiwatch Mini

Publication Date: April 28, 2015


Technical note: Validation of an automatic recording system to assess behavioural activity level in sheep (Ovis aries)

The welfare of an individual can be assessed by monitoring behavioural changes, such as inactivity, that may indicate injury or disease. In this study we validated the Actiwatch Mini® activity monitor (AM) for automatic recording of behavioural activity levels of nine Texel ewes. The AM devices were attached to collars placed around the necks of […]

Related Product: Actiwatch Mini

Publication Date: April 15, 2015


Different daily patterns of serum cortisol and locomotor activity rhythm in horses under natural photoperiod

The goal of the present study was to investigate the daily pattern of serum cortisol rhythm in relation to the total locomotor activity in horses housed in an individual box under a 12-hour light-dark cycle. Blood samples were collected at 3-hour intervals over a 48-hour period via an intravenous cannula inserted into the jugular vein […]

Related Product: Actiwatch Mini

Publication Date: April 1, 2015


Meal size and feeding management strategies influence the daily rhythm of total locomotor activity in horses (Equus caballus)

Keeping and management of horses can induce changes to instinctive and innate behavioural patterns. We investigated the effect of five different management conditions in five groups of horses. All groups were housed in individual boxes under natural environmental and lighting conditions. They were fed three times a day (07:00, 12:30 and 20:00) and had free […]

Related Product: Actiwatch Mini

Publication Date: March 30, 2015


Total locomotor activity and serum cortisol levels: daily rhythm in dogs

No abstract

Related Product: Actiwatch Mini

Publication Date: October 20, 2014


Early and progressive circadian abnormalities in Huntington’s disease sheep are unmasked by social environment

Insidious changes in behaviour herald the onset of progressive neurodegenerative disorders such as Huntington’s disease (HD), sometimes years before overt symptoms are seen. Sleep and circadian disturbances are particularly disruptive symptoms in patients with neurological disorders, but they are difficult to measure in humans. Here we studied circadian behaviour in transgenic HD sheep expressing the […]

Related Product: Actiwatch Mini

Publication Date: July 1, 2014


Comparison of daily distribution of rest/activity in companion cats and dogs

The aim of this study was to characterize cats and dogs’ locomotor activity patterns in relation to housing conditions and owner interaction. Five adult cats and five adult dogs belonging to ten different owners were enrolled in the study. Animals were housed in apartments having daily access to the garden. Total locomotor activity (TLA) was […]

Related Product: Actiwatch Mini

Publication Date: March 17, 2014


Effect of housing conditions and owner’s schedule on daily total locomotor activity in dogs (Canis familiaris).

The purpose of this study was to evaluate the influence of housing conditions on daily rhythm of total locomotor activity in dogs. Seven clinically healthy dogs (Canis familiaris) were used and divided into two groups: Group A lived with its owner in indoor environment, Group B housed in individual box of 140 × 200 cm. Each dog was […]

Related Product: Actiwatch Mini

Publication Date: February 4, 2013


Influence of time of food administration on daily rhythm of total locomotor activity in ponies

The activity rhythm can be entrained by nonphotic cues, especially food availability. Daily feeding schedules can act as “Zeitgeber” to synchronize the circadian system. In this study, the authors evaluated the influence of different feeding schedules on the daily rhythm of total locomotor activity in ponies. Six clinically healthy Shetland ponies were kept under natural […]

Related Product: Actiwatch Mini

Publication Date: February 1, 2013


Daily rhythm of total activity pattern in domestic cats (Felis silvestris catus) maintained in two different housing conditions

Housing conditions and human presence have been shown to have an influence on daily amount of activity in herbivores. Only few studies on the behavior of cats and interactions between cat and human were conducted, and these few works are referred to cats housed in laboratories. At the same way, only few and conflicting reports […]

Related Product: Actiwatch Mini

Publication Date: January 5, 2013


Toll-Like Receptor Agonists as Antecedent Therapy for Ischemic Brain Injury: Advancing Preclinical Studies to the Nonhuman Primate

Antecedent therapy for ischemic brain injury has the potential to protect a large, high-risk patient population from the devastating effects of cerebral ischemia associated with cardiac surgery. Substantial evidence has shown that preconditioning with a modestly damaging stimulus induces powerful endogenous neuroprotection. Pharmacological agents that stimulate toll-like receptors (TLRs) induce robust neuroprotective effects as preconditioning […]

Related Product: Actiwatch Mini

Publication Date: March 2, 2012


Effect of different farming management on daily total locomotor activity in sheep

The aim of this study was to investigate the influence of different housing conditions and feeding schedules on the daily rhythm of total locomotor activity (TLA) in sheep. A total of 15 sheep, divided into 3 equal groups, were used. Group A was kept in a grazing area, group B in a box and was […]

Related Product: Actiwatch Mini

Publication Date: June 22, 2011


Comparison between circadian motor activity in pony and horse

In this study we investigated the influence of body mass on the daily rhythm of total motor activity in ponies and horses. Six clinically healthy Shetland pony breed with a body mass of 180 ± 30 kg and six healthy horses Sella Italiana breed with a body mass of 530 ± 20 kg were used. […]

Related Product: Actiwatch Mini

Publication Date: June 1, 2011


Daily rhythms of rectal temperature and total locomotor activity in trained and untrained horses

In this study the authors evaluated the influence of physical activity on the daily rhythm of rectal temperature and total locomotor activity in untrained and trained horses. Rectal temperature and locomotor activity of 12 Italian saddle horses, 6 untrained (group A) and 6 trained (group B), was recorded for 48 h. Rectal temperature was recorded […]

Related Product: Actiwatch Mini

Publication Date: February 24, 2011


Nycthemeral rhythms of total locomotor activity and oxidative markers in horse

The aim of this study was to investigate the nycthemeral rhythm of total locomotor activity (TLA) in horse and the possible involvement of the daily organization of rest/activity cycles in the fluctuation of the redox state. For this purpose we recorded TLA and determined oxidative markers in ten clinically healthy Italian Saddle horses. TLA was […]

Related Product: Actiwatch Mini

Publication Date: February 18, 2011


A comparison of daily total locomotor activity between the lactation and the dry period in dairy cattle

The objective of this study was to investigate the influence of farming management on the total locomotor activity (TLA) behaviour in dairy cattle. We recorded 24 h/day TLA in five not pregnant Holstein Friesian cows during parts of the lactation and dry periods, by means of an activity monitoring system (Actiwatch mini®) for seven days […]

Related Product: Actiwatch Mini

Publication Date: February 1, 2011


The effect of photic entrainment and restricted feeding on food anticipatory activity in Ovis aries

The aim of this study was to investigate the influence of light/dark (L/D) cycle, associated with restricted feeding, on total daily locomotor activity in sheep. Eight clinically healthy Comisana ewes were housed individually in a soundproof box and subjected to three different light and feeding periods. During period 1 (light on 8:00; light off 20:00) […]

Related Product: Actiwatch Mini

Publication Date: August 21, 2010


Circadian rhythms of redox states and total locomotor activity in dairy cattle

We want to study the circadian rhythm of dROMs and anti-oxidative power in dairy cattle during dry period and the possible involvement of the circadian organization of rest/activity cycles in the fluctuation of redox state. For this purpose we recorded TLA in five clinically healthy Bruna Italian dairy cattle by means of an actigraphy-based data […]

Related Product: Actiwatch Mini

Publication Date: February 1, 2010


Photic and non‐photic entrainment on daily rhythm of locomotor activity in goats

We studied the photic (L/D cycle) and non‐photic (restricted feeding) entrainment on the patterns of daily rhythm of total locomotor activity in goats. Six female Maltese goats were subjected to three different artificial L/D cycles: 12/12 L/D, 12/12 D/L and constant light. During the 12/12 L/D and 12/12 D/L, food and water were available ad […]

Related Product: Actiwatch Mini

Publication Date: January 28, 2010


Evaluation of total locomotor activity and oxidative markers daily rhythms in sheep

For our study, five clinically healthy Comisana sheep, housed individually in a soundproof light-tight box, were exposed to artificial photoperiod (12/12 light/dark). Locomotor activity was continuously recorded and blood samples were collected every 3 h over a 24 h period. On blood samples, reactive oxygen metabolites (dRoms), antioxidant barrier (Oxy-ads) and thiol-antioxydant barrier (SHp) were […]

Related Product: Actiwatch Mini

Publication Date: January 4, 2010


Daily locomotor activity in five domestic animals

The aim of this study was to compare locomotor activity in five domestic animals. Five female Comisana sheep, five female Maltese goats, five female Bruna cows, and five female Thoroughbred horses were housed separately in a soundproof box equipped with an airflow system and five female Blue Vienna rabbits were individually housed in metallic cages. […]

Related Product: Actiwatch Mini

Publication Date: January 1, 2010


Daily rhythms of 25 physiological variables in Bos taurus maintained under natural conditions

To further understanding of the multiple temporal relationships of the physiological process, we monitored simultaneously 25 different variables in individual cows. We used 6 Bruna Italiana non – pregnant and non – lactating cows from the same farm. The animals were housed individually in a 12 m2 box under natural 14/10 light/dark cycle. They were […]

Related Product: Actiwatch Mini

Publication Date: March 15, 2009


Seasonal change of daily motor activity rhythms in Capra hircus

To evaluate if seasonal changes in photoperiod and temperature were associated with changes in total daily motor activity we recorded the total daily motor activity of five clinically healthy goats at four different times of the year (vernal equinox, summer solstice, autumn equinox and winter solstice). Goats were housed under natural photoperiod and natural ambient […]

Related Product: Actiwatch Mini

Publication Date: September 1, 2008


Circadian activity rhythm in sheep and goats housed in stable conditions

The aim of this study was to establish the circadian rhythm of total activity in sheep and goats kept in housed conditions. For our study five Comisana breed sheep and five Maltese breed goats were kept in an individual box of 12 m2 under artificial 12/12 light/dark cycle for 14 days. We equipped the animals […]

Related Product: Actiwatch Mini

Publication Date: June 4, 2008


Locomotor activity and serum tryptophan and serotonin in goats: daily rhythm

The goal of the present study was to investigate the daily amount of motor activity and the daily rhythm of tryptophan and serotonin in goats housed in individual boxes under a 12/12 light/dark cycle. We equipped six Girgentana breed goats with Actiwatch-Mini® (Cambridge Neurotechnology Ltd., UK), actigraphy-based data loggers that record a digitally integrated measure […]

Related Product: Actiwatch Mini

Publication Date: April 22, 2008


Seasonal variations in daily rhythms of activity in athletic horses

Circadian rhythms reflect extensive programming of biological activity that meets and exploits the challenges and opportunities offered by the periodic nature of the environment. In the present investigation, we recorded the total activity of athletic horses kept at four different times of the year (vernal equinox, summer solstice, autumn equinox and winter solstice), to evaluate […]

Related Product: Actiwatch Mini

Publication Date: March 28, 2008


Daily rhythms of activity in horses housed in different stabling conditions

Total locomotor activity was studied in 10 Thoroughbreds housed under a natural 12/12 light/dark cycle. Five horses were housed in individual boxes, and five were housed in individual boxes with a paddock. In order to record locomotor activity, on each horse was placed an Actiwatch-Mini® (Cambridge Neurotechnology Ltd, UK), actigraphy-based data loggers that record a […]

Related Product: Actiwatch Mini

Publication Date: January 1, 2008


Effects of restricted feeding on circadian activity rhythms of sheep—A brief report

The purpose of the present experiment was to investigate the impact of timed restricted feeding on the behavioural circadian organization of sheep. For this purpose, total activities of sheep subjected both to 12-h light:12-h dark (12:12 LD) cycle and to periodic food-availability during the dark phase were recorded. When food was available ad libitum, sheep […]

Related Product: Actiwatch Mini

Publication Date: November 1, 2007


A novel method for activity monitoring in small non-human primates

Patterns of spontaneous activity are valuable reflections of well-being in animals and humans and, because of this, investigations have frequently incorporated some form of activity monitoring into their studies. It is widely believed that activity monitoring, alongside assessments of general behaviour, should be included in initial CNS safety pharmacology screening. As the number of marmoset […]

Related Product: Actiwatch Mini

Publication Date: April 1, 2005


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